Custom target RNA Seq MagIC Beads

Enrich custom RNA targets up to 100 000x over the most abundant non-target RNA molecules, directly from the biological samples.

Description

Reproducible, fast, reliable RNA capture

RNA enrichment with Magnetic Instant Capture Beads (MagIC Beads) is the best option for:

  • Pulldown and analysis of lncRNA.
  • Large scale isolation from cell lysates.
  • Epigenetic modification studies.
  • Targeted RNA sequencing with NGS and 3rd Generation Sequencing.
  • …and more

Unmatched enrichment levels

RNA Seq MagIC Beads - MALAT 1 enrichment

Total RNA isolated from HEK293 cells was incubated with MagIC Beads targeting human GAPDH, LINC00086 and MALAT1 transcripts for 30min at 55°C. RNA attached to the beads was washed, eluted and subjected to cDNA synthesis. Levels of 18S rRNA, GAPDH, ACTB and MALAT1 were measured with RT-qPCR. The target RNA was enriched from over 10 000 to over 100 000 fold over non-target transcripts, by MALAT1 targeting MagIC Beads, but not by beads targeting other transcripts. Results from two biological replicates are shown.

 

RNA Seq SARS-CoV-2 MagIC Beads enrichment levels

1100ng of total RNA isolated from HEK293 cells was spiked-in with 0.5ng of SARS-CoV-2 in-vitro transcribed RNA and incubated with MagIC Beads targeting SARS-CoV-2 genome for 30min at 60°C. RNA attached to the beads was washed, eluted, and subjected to cDNA synthesis. Levels of GAPDH, ACTB, 18S rRNA, and SARS-CoV-2 were measured with RT-qPCR. The target RNA was enriched from over 1 000 to over 10 000 fold over non-target transcripts, by SARS-CoV-2 targeting MagIC Beads.

Advantages of RNA Seq MagIC Beads – Custom target:

  • High efficiency and specificity:
    • Isolate the target of interest instead of creating a generic RNA pool.
    • Enrich the target RNA up to 100 000x.
    • Capture up to 100% of the target molecule.
    • No molecular tag related biases.
    • Reproducible and consistent levels of enrichment.
    • Purification can take place under very strong denaturing conditions, unlike biotin based approaches.
  • Simple and time-saving protocol:
    • Only 3 steps to obtain enriched RNA samples.
    • Below 10 minutes of hands-on time.
    • The whole procedure takes less than 1 hour.
    • No additional components needed.
  • Custom design for any RNA molecule:
    • Target any transcript or pool of transcripts.

A simple workflow for RNA enrichment

RNA Seq MagIC Beads workflow

How it works

RNA enrichment with MagIC (Magnetic Instant Capture) Beads can be employed when specific RNA is to be analyzed without the accompanying context of all cellular RNAs. This can be used for targeted sequencing with 2nd or 3rd generation sequencing technologies as MagIC Beads RNA enrichment is downstream application-agnostic.

The targeted sequencing approach is especially useful for low copy number transcripts, which cannot be efficiently sequenced without enrichment or in case of more typical transcripts simply to reduce overall costs of sequencing when only a defined portion of the transcriptome is to be investigated. This approach can be employed in the study of the exact sequence of transcripts in question, the study of alternative polyadenylation sites, alternative transcription start sites, splicing variants, sequence variations, or RNA modifications.

The enrichment can also be combined with low throughput biochemical methods to study RNA of interest in cases when the complexity of an RNA sample is interfering with the analysis. This can be an issue when low copy number transcripts are investigated even when standard, well-established methodologies are being employed. MagIC Beads provide a viable solution to this problem.

Additional information

  • Supplied with beads and hybridization buffer.
  • Shipping condition: room temperature.
  • Storage Temperature: 4°C.
  • Durability: 60 months from date of manufacture.
  • Protocol